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[Flow laser cytometry for evaluation of the human immune system]
B V Pinegin1, A A Iarilin, D V Mazurov
1State Research Center-Institute of Immunology, Moscow, Russia.
Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
|January 1, 2003
Summary
Flow laser cytometry (FLC) analyzes immune cells by examining markers, functions, and activities. This review covers FLC techniques for assessing lymphocyte phenotyping, cellular functions, and immune responses.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Context:
- Flow laser cytometry (FLC) is a key technology for immune cell analysis.
- Phenotyping lymphocytes by expressed markers is a common application.
- FLC enables comprehensive evaluation of immunocompetent cell functions.
Purpose:
- To review various flow laser cytometry techniques for immune system cell studies.
- To highlight FLC's utility in assessing cellular functions and immune responses.
- To introduce novel FLC-based tests for immune cell analysis.
Summary:
- FLC techniques are reviewed for immune cell studies, including lymphocyte phenotyping.
- Functional parameters like ingestive/microbicidal activity, calcium mobilization, and cytokine identification are assessed.
- Proposed tests cover lymphocyte proliferation, natural killer cytotoxicity, apoptosis, and monocyte/macrophage protein processing.
Impact:
- Provides a comprehensive overview of FLC applications in immunology.
- Facilitates deeper understanding of immune cell behavior and function.
- Offers advanced methods for immune system research and diagnostics.