[Construction of adenovirus type 7(vaccine strain) vector and expression of beta-galactosidase gene]

Human adenovirus type 7 DNA was extracted from purified virus cultured in WI-38 cells. The essential fragment (68-100 mu) of Ad7 DNA was used for constructing a non-defective Ad7 vector termed as pAd7 delta E3. The vector was characterized by a deletion at the E3 region that contains a multicloning site for the insertion of foreign genes. A helper-independent adenovirus type 7-beta-galactosidase recombinant was established. The recombinant contains the beta-galactosidase gene flanked by CMV early promoter and SV40 poly A signal. The constructed recombinant virus showed efficient capacity in expressing the foreign gene, beta-galactosidase.