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[HSP70 expression of middle ear mucosa in acute suppurative otitis media]
1PLA Otolaryngological Diseases Center, Bethune International Peace Hospital, Shijiazhuang 050082.
Objective:
To study HSP70 expression of middle ear mucosa in acute suppurative otitis media.
Method:
An animal model of acute middle ear infection was established by inoculating Klebsiella Pneumoniae into middle ear of guinea pigs. Animals were sacrificed 1, 3, 5 and 7 days after inoculation. The expression of Hsp70 epitope related proteins in middle ear mucosa were analysed by modified western blot test.
Result:
Very light 70 kD bands were recognized by anti Hsp70 monoclonal antibody in the unstressed group. However, middle ear mucosa of the stressed group not only showed relatively strong 70 kD bands, but also expressed more intensely 31 kD and 17 kD bands. The infected mucosa expressed one 70 kD band at 1st day, the strongest band at 3rd day, falling at 5th day; 31 kD was recognized two bands at 3rd day, the strongest bands at 5th day, falling at 7th day; two bands of 17 kD were detected in all days, with the strongest bands at 7th day.
Conclusion:
The results suggested that middle ear mucosa expressed Hsp70 and Hsp70 epitope related proteins like 31 kD and 17 kD in acute otitis media.