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Updated: Jul 18, 2026
Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
[Plasma autoantibodies to heat shock proteins in idiopathic sensorineural hearing loss]
1Department of Otorhinolaryngology, Central Hospital of Xiangfan, Hubei 441021.
Objective:
To identify the specificity of autoantibodies to heat shock proteins in idiopathic sensorineural hearing loss (ISNHL) and to evaluate the existence of autoantibodies.
Method:
Plasma samples were obtained from patients with ISNHL (n = 41), systemic lupus erythematosus (n = 22), and normal controls (n = 30). Plasma autoantibodies to heat shock proteins (HSPs) were detected by Western blot using purified recombinant human HSP27, HSP60, HSP71 HSP90 alpha and HSP90 beta as antigen respectively.
Result:
In the ISNHL, the incidence of plasma autoantibodies to recombinant human HSP27, HSP60, HSP71, HSP90 alpha and HSP90 beta were 12.1%, 7.3%, 51.2%, 4.8% and 0% respectively. The incidence of autoantibody to HSP71 was significantly greater than that in systemic lupus erythematosus and in normal controls (P < 0.01).
Conclusion:
These results indicated that the autoantibody to HSP71 was main autoantibodies to HSPS in ISNHL and its presence might serve as a marker for an autoimmune etiology of these patients' hearing loss.
