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Related Experiment Videos

In vivo molecular-genetic imaging.

Ronald G Blasberg1, Juri Gelovani-Tjuvajev

  • 1Department of Neurology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA. blasberg@neuro1.mskcc.org

Journal of Cellular Biochemistry. Supplement
|January 29, 2003
PubMed
Summary
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Noninvasive molecular imaging uses reporter genes and probes to track gene expression and biological processes in vivo. This technology offers a non-sampling method to study diseases in animal models and potentially humans.

Area of Science:

  • Molecular biology
  • Medical imaging
  • Genetics

Background:

  • Noninvasive in vivo molecular imaging, including PET and MRI, has advanced significantly.
  • Current strategies often use "indirect" imaging with reporter genes and probes to assess gene expression levels.
  • Reporter gene expression can be constitutive or inducible, allowing for monitoring of gene activity or controlled gene expression.

Purpose of the Study:

  • To review examples of imaging endogenous biological processes in animals using reporter constructs and PET.
  • To highlight the value of non-invasive assays for studying molecular-genetic and cellular processes.
  • To emphasize the spatial and temporal insights gained from non-invasive imaging for disease understanding.

Main Methods:

  • Utilizing reporter gene constructs coupled with complementary reporter probes for indirect imaging.

Related Experiment Videos

  • Employing nuclear imaging techniques like Positron Emission Tomography (PET) with radiolabeled probes.
  • Reviewing applications in imaging transcriptional and post-transcriptional regulation, protein-protein interactions, and promoter activity.

    • Main Results:

    • Demonstrated successful in vivo imaging of various biological processes, including gene regulation and protein interactions.
    • Showcased the ability to monitor gene (vector) activity, duration, and tissue-specific expression.
    • Highlighted the potential for imaging weak promoters and cis- vs. trans-reporter configurations.

    Conclusions:

    • Non-invasive molecular imaging provides valuable spatial and temporal data complementary to traditional ex vivo methods.
    • Versatile and sensitive non-invasive assays are crucial for monitoring molecular-genetic and cellular processes in disease models.
    • Future applications hold promise for both animal research and human studies, advancing disease understanding.