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[Use PCR technique to detect mitochondrial DNA in rat membranous labyrinth]
Objective:
To establish a sensitive and reliable method of mtDNA detection in the membranous labyrinth of rat inner ear.
Method:
A mtDNA segment of 601 bp containing the genes which encode ND1 subunit 16S rRNA in rat inner ear membranous labyrinth was detected by PCR method. The method is compared with Edris's mtDNA isolation method.
Result:
A 601 bp PCR product of mtDNA in the membranous labyrinth of rat ear inner can be obtained by the two methods. But the requirement of the sample quantities was different for the two methods. The extraction of mtDNA need two of inner ears membranous labyrinth to get reliably result by the method adapted from Seidman. However, the mtDNA extraction procedure from Edris's method need six of inner ears of the in order to get reliably result.
Conclusion:
The method of mtDNA isolation, amplification and detection membranous labyrinth of rat inner ear by PCR method adapted from seidman is more sersitive.