Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A conductometric biosensor for biosecurity.

Zarini Muhammad-Tahir1, Evangelyn C Alocilja

  • 1Biosystems Engineering, Michigan State University, East Lansing, MI 48824, USA.

Biosensors & Bioelectronics
|April 23, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proof-of-Concept Nanoparticle-Based Biosensor for Detecting the African Swine Fever Virus Across Multiple Genotypes Using In Silico and In Vitro Approaches.

ACS omega·2025
Same author

Biosensor Strategies for Rapid African Swine Fever Virus Detection.

ACS omega·2025
Same author

Integrated Sample to Detection of Carbapenem-Resistant Bacteria Extracted from Water Samples Using a Portable Gold Nanoparticle-Based Biosensor.

Sensors (Basel, Switzerland)·2025
Same author

A Review of the Diagnostic Approaches for the Detection of Antimicrobial Resistance, Including the Role of Biosensors in Detecting Carbapenem Resistance Genes.

Genes·2025
Same author

Genomic nano-biosensor for rapid detection of the carbapenem-resistant gene <i>bla</i> <sub>NDM-1</sub> in carbapenemase-producing bacteria.

Nanoscale advances·2025
Same author

Parallel Detection of the Unamplified Carbapenem Resistance Genes <i>bla</i><sub>NDM-1</sub> and <i>bla</i><sub>OXA-1</sub> Using a Plasmonic Nano-Biosensor with a Field-Portable DNA Extraction Method.

Biosensors·2025
Same journal

GLASS-seq: a gel-anchored, ligation-assisted, scalable biosensing platform for low-cost regional spatial transcriptomics.

Biosensors & bioelectronics·2026
Same journal

CRISPR/Cas12a-based dual-modal signal platform using MIL-101(Fe) for colorimetric and electron spin resonance detection of HPV-16 nucleic acid.

Biosensors & bioelectronics·2026
Same journal

Fully automated centrifugal microfluidic system for self-calibrating isothermal nucleic acid quantification.

Biosensors & bioelectronics·2026
Same journal

Synergistic mode-field pre-expansion and geometric compression in hetero-structured microfibers for ultrasensitive glucose sensing.

Biosensors & bioelectronics·2026
Same journal

An amplification-free dual-readout biosensor integrating colorimetry and single-particle counting for ultrasensitive miRNA detection in esophageal cancer.

Biosensors & bioelectronics·2026
Same journal

An all-in-one microfluidic system via data-driven design for on-site genotyping of genetically modified foods.

Biosensors & bioelectronics·2026
See all related articles

A novel conductometric biosensor offers rapid and sensitive detection of foodborne pathogens like E. coli O157:H7. This immunosensor technology provides near real-time results for improved food safety and biosecurity.

Area of Science:

  • Biosensor technology
  • Food safety analysis
  • Immunochemical detection

Background:

  • Foodborne pathogens pose significant risks to public health and biosecurity.
  • Current detection methods can be time-consuming and require specialized laboratory equipment.
  • There is a need for rapid, sensitive, and field-deployable detection systems.

Purpose of the Study:

  • To develop and evaluate a conductometric biosensor for the detection of foodborne pathogens.
  • To demonstrate the biosensor's specificity and sensitivity for key pathogens.
  • To establish a near real-time detection mechanism for biosecurity applications.

Main Methods:

  • Development of a conductometric biosensor integrating an electrochemical sandwich immunoassay with a lateral flow system.

Related Experiment Videos

  • Utilizing antibody specificity for pathogen capture and electrochemical signal transduction.
  • Performance evaluation using enterohemorrhagic Escherichia coli O157:H7 and Salmonella spp.

    • Main Results:

    • The biosensor demonstrated specific and sensitive detection capabilities.
    • Achieved a lower limit of detection of approximately 7.9 x 10^1 colony forming units per milliliter.
    • Detection process completed within 10 minutes, indicating near real-time analysis.

    Conclusions:

    • The developed conductometric biosensor is a promising tool for rapid foodborne pathogen detection.
    • Its adaptable antibody specificity allows for potential application against a broader range of pathogens.
    • This technology can enhance food safety monitoring and biosecurity measures.