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Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue
Published on: June 14, 2020
[Some features of tonsillar microflora and microbial interaction (in normal and pathological states)]
O V Bukharin1, B Ia Usviatsov, L M Khusnutdinova
1Research Institute of Cellular and Intracellular Symbiosis, Medical Academy, Orenburg, Russia.
Abstract:
The description of a new method for the determination of interaction between bacterial strains isolated from the mucous membrane of tonsils of healthy children and patients with chronic tonsillitis. In the analysis of mutual influence of symbionts on the hemolytic, lecitovitellase, lysozyme and antilysozyme activity of microbes isolated from healthy children no changes in the expression of these pathogenicity and persistence factors have been detected in the overwhelming majority of cases (70.8-89.9%). In the microflora of sick children interactions were synergic with respect to pathogenicity factors in 35.2-46.7% of cases and antagonistic in 18.7-31.3% of cases.
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