Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RNA crystallization.

Barbara L Golden1, Craig E Kundrot

  • 1Department of Biochemistry, Purdue University, 175 S. University Street, West Lafayette, Indiana 47907, USA.

Journal of Structural Biology
|April 30, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA-Puzzles Round IV: 3D structure predictions of four ribozymes and two aptamers.

RNA (New York, N.Y.)·2020
Same author

The NASA Twins Study: A multidimensional analysis of a year-long human spaceflight.

Science (New York, N.Y.)·2019
Same author

Divalent Metal Ion Activation of a Guanine General Base in the Hammerhead Ribozyme: Insights from Molecular Simulations.

Biochemistry·2017
Same author

Two Active Site Divalent Ions in the Crystal Structure of the Hammerhead Ribozyme Bound to a Transition State Analogue.

Biochemistry·2015
Same author

Two Divalent Metal Ions and Conformational Changes Play Roles in the Hammerhead Ribozyme Cleavage Reaction.

Biochemistry·2015
Same author

Thio effects and an unconventional metal ion rescue in the genomic hepatitis delta virus ribozyme.

Biochemistry·2013
Same journal

MLAC: MicroED-assisted ligand structure analysis in complexes and its application to hERG-ligand complexes.

Journal of structural biology·2026
Same journal

Ultrastructural evidence of autophagy-related processes and mitochondrial remodeling in the myxozoan parasite Henneguya piaractus.

Journal of structural biology·2026
Same journal

Architecture and dynamics of a supramolecular oxygen transport system in human homogentisate 1,2-Dioxygenase.

Journal of structural biology·2026
Same journal

Connecting pathways between mineralized fibrocartilage and bone at the Achilles tendon insertion.

Journal of structural biology·2026
Same journal

Structural and functional characterization of thermostable EstS1 esterase for BHET degradation.

Journal of structural biology·2026
Same journal

Following the white rabbit: multiscale 2D3D correlative imaging of bone structure.

Journal of structural biology·2026
See all related articles

RNA crystallization techniques are advancing, enabling detailed structural analysis. Researchers can now design, synthesize, and purify RNA molecules for structural studies using methods adapted from protein crystallography.

Area of Science:

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Advancements in RNA synthesis and purification technologies have fueled progress in RNA structure determination.
  • RNA crystallization methods are adapted from established protein crystallography techniques.

Purpose of the Study:

  • To review techniques for designing, synthesizing, purifying, and crystallizing RNA molecules.
  • To highlight considerations unique to or crucial for RNA crystallization.

Main Methods:

  • Rational sequence design to enhance crystallizability and prevent alternative structures.
  • Chemical solid-state synthesis or enzymatic production using RNA polymerase.
  • Purification via High-Performance Liquid Chromatography (HPLC) or gel electrophoresis.

Related Experiment Videos

  • Crystallization typically achieved through vapor diffusion methods.

    • Main Results:

    • The availability of high-quality RNA has led to significant growth in RNA structure research.
    • Established protein crystallography approaches are applicable to RNA crystallization.
    • Specific considerations for RNA sequence design and purification are critical.

    Conclusions:

    • RNA crystallization is a viable and increasingly powerful method for elucidating RNA structures.
    • The described techniques facilitate the study of RNA molecules at a structural level.
    • Further exploration of RNA crystallization is essential for advancing molecular biology.