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[Proteomics analysis and biochemical activity detection on facial nerve regeneration fluid]
Hui Yang1, Chuanyu Liang, Bingqing Gao
1Department of Otolaryngology, Huaxi Hospital of Sichuan University, Chengdu 610041.
Objective:
To explore the protein components and the biological neutrional activity upon motor neuron, so that take a general, basic study on the nerve regeneration microenvironment which be important to facial nerve regeneration.
Method:
The animal model of rabbit facial regeneration microenvironment is established. Advanced technology of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) was used to study on the structural proteins in the regeneration fluid, as well as the motor neuron culture was used to test the biological activity in it.
Result:
850 +/- 78 protein spots were detected in the regeneration fluid. The body area, neurite length and OD value of experimental group are higher than that of control group.
Conclusion:
We got the protein image of facial nerve regeneration fluid by 2-D PAGE, and proved that many kinds and number of proteins in the regeneration fluid have neutrional activity on motor neuron.