Dynamics of precursor cell composition in bone marrow culture derived from mice deficient by tumor necrosis factor

Hemopoiesis in a long-term bone marrow culture derived from mice deficient for tumor necrosis factor was maintained for more than 130 weeks, which was 4 times longer than in cultures from wild type mice. The dynamics of hemopoietic precursor cells of different maturity was studied in the suspension fraction of the culture. The incidence of granulocyt-macrophage precursor cells and cell forming cobblestone areas was studied by the method of limiting dilutions in culture. In contrast to cultures derived from wild type mice, in long-term bone marrow culture derived from tumor necrosis factor deficient mice, first, the incidence of early precursor cells gradually increased and then the incidence of all precursor cells sharply increased.