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Evaluation of the disc diffusion method with a comparison study for fluconazole susceptibility of Candida strains
Semra Kustimur1, Ayse Kalkanci, Halil Mansuroglu
1Gazi University Faculty of Medicine, Department of Microbiology and Clinical Microbiology, Besevler, 06500, Ankara, Turkey.
Objective:
To performance susceptibility testing of antifungal agents. Due to the increasing number of resistant strains, susceptibility testing of antifungal agents is gaining importance.
Methods:
We compared the results of standard macrotube dilution reference method with two different microdilution methods, as well as the disc diffusion method in order to test the susceptibility of 150 Candida strains to fluconazole.
Results:
Overall correlation between microdilution and macrodilution methods was 86%. It was 91% between the Minimal Inhibitory Concentrations obtained from macrodilution and disc diffusion zone diameters.
Conclusion:
The disc diffusion test was evaluated as a low-cost, reproducible, and efficient way of assessing the in vitro susceptibility of Candida strains to fluconazole.