Related Experiment Video
Updated: Jul 20, 2026

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
Published on: June 19, 2013
Rapid identification of Candida species by using nuclear magnetic resonance spectroscopy and a statistical
Uwe Himmelreich1, Ray L Somorjai, Brion Dolenko
1Centre for Infectious Diseases and Microbiology, Institute for Clinical Pathology and Medical Research, University of Sydney at Westmead Hospital, Westmead, New South Wales 2145, Australia. uwe@med.usyd.edu.au
Abstract:
Nuclear magnetic resonance (NMR) spectra were acquired from suspensions of clinically important yeast species of the genus Candida to characterize the relationship between metabolite profiles and species identification. Major metabolites were identified by using two-dimensional correlation NMR spectroscopy. One-dimensional proton NMR spectra were analyzed by using a staged statistical classification strategy. Analysis of NMR spectra from 442 isolates of Candida albicans, C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis resulted in rapid, accurate identification when compared with conventional and DNA-based identification. Spectral regions used for the classification of the five yeast species revealed species-specific differences in relative amounts of lipids, trehalose, polyols, and other metabolites. Isolates of C. parapsilosis and C. glabrata with unusual PCR fingerprinting patterns also generated atypical NMR spectra, suggesting the possibility of intraspecies discontinuity. We conclude that NMR spectroscopy combined with a statistical classification strategy is a rapid, nondestructive, and potentially valuable method for identification and chemotaxonomic characterization that may be broadly applicable to fungi and other microorganisms.
More Related Videos
Related Concept Videos
Applications Of NMR In Biology
The...
Methods of Classification and Identification
Modern Molecular Taxonomy
Rapid Identification of Pathogens

