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Trace element speciation by ICP-MS in large biomolecules and its potential for proteomics
Alfredo Sanz-Medel1, María Montes-Bayón, María Luisa Fernández Sánchez
1Department of Physical and Analytical Chemistry, University of Oviedo, C/ Julián Clavería 8, 33006 Oviedo, Spain. asm@sauron.quimica.uniovi.es
Analytical and Bioanalytical Chemistry
|August 9, 2003
Summary
This study reviews analytical methods for trace element speciation in biomolecules. Advanced hyphenated techniques, like ICP-MS coupled with chromatography, are key for understanding metal-biomolecule interactions in biological and clinical settings.
Area of Science:
- Bioinorganic Chemistry
- Analytical Chemistry
- Clinical Chemistry
Background:
- Trace elements form crucial associations with large biomolecules.
- Understanding these metal-biomolecule interactions is vital in bioinorganic and clinical fields.
- Speciation analysis of these complexes in biological fluids presents significant complexity.
Purpose of the Study:
- To critically review analytical strategies and instrumentation for trace element speciation in biomolecules.
- To highlight the capabilities of hyphenated techniques, particularly ICP-MS, for real-life speciation analysis.
- To assess the potential of current speciation knowledge for "heteroatom-tagged" proteomics.
Main Methods:
- Review of analytical strategies for speciation analysis.
- Evaluation of state-of-the-art instrumentation.
- Focus on hyphenated techniques coupling chromatography (SEC, IE, RP, CE) with ICP-MS detection.
Main Results:
- Hyphenated techniques with ICP-MS are potent tools for speciation analysis.
- Successful separation of metallo-complexes with proteins, metallothioneins, selenoproteins, DNA, and carbohydrates reported.
- Established methods for analyzing metal and semimetal speciation in large biocompounds.
Conclusions:
- ICP-MS coupled with chromatography offers realistic and powerful solutions for trace element speciation in biomolecules.
- These techniques are essential for advancing our understanding of bioinorganic and clinical implications.
- Potential exists for "heteroatom-tagged" proteomics using these advanced speciation methods.