GABAergic system in the anxiolytic effect of Proproten: experimental study

The medicinal preparation Proproten contains ultralow doses of antibodies to S100 protein that acts as an important regulator of integrative activity in the brain and synaptic processes. Intracerebroventricular administration of Proproten, diazepam, and mexidol in doses of 2.5 ml/kg, 2 mg/kg, and 100 mg/kg, respectively, produced a strong anxiolytic effect on male outbred rats in the conflict situation and markedly increased the incidence of punished drinking. Antagonists of GABAergic transmission bicuculline (GABAA receptor blocker) and picrotoxin (chlorine channel blocker) produced the pro-conflict anxiogenic effect, which was accompanied by a decrease in the number of punished drinking in control animals. The anti-conflict effect of Proproten was less pronounced during blockade of GABAA receptors or chlorine channels. Bicuculline and picrotoxin similarly modulated the anxiolytic effect of diazepam and mexidol. Our results suggest that the GABAergic system plays a role in the anxiolytic effect of diazepam, mexidol, and Proproten.