Related Experiment Video
Updated: Sep 14, 2026
A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
Published on: October 14, 2016
Electron microscope study of a cell-free induced leukemia of the mouse: a preliminary report
Abstract:
Preliminary results of an electron microscope study of a leukemia of the mouse transmissible by cell-free filtrates are reported. In twelve out of forty-seven specimens examined, virus-like particles were observed. So far, these particles have not been observed in control, non-leukemic animals. They are located inside the cytoplasm of leukemic cells which infiltrate the spleen or the liver. Their diameter is approximately 78 mmicro. Their resemblance to other particles recently described in different mouse tumors is stressed. Their significance and, in correlation with ultrafiltration data, their possible etiological meaning are discussed.
Insights
Researchers observed virus-like particles in mouse leukemia specimens. These particles, found in leukemic cells, were absent in healthy controls, suggesting a potential role in the disease.
Area of Science:
- Oncology
- Virology
- Electron Microscopy
Background:
- Leukemia is a significant health concern in mouse models.
- Transmissible agents are implicated in the development of certain leukemias.
- Electron microscopy is crucial for visualizing sub-cellular structures.
Purpose of the Study:
- To investigate the presence of virus-like particles in transmissible mouse leukemia.
- To characterize the morphology and location of observed particles.
- To explore the potential etiological significance of these particles.
Main Methods:
- Electron microscopy was used to examine spleen and liver tissues from leukemic mice.
- Cell-free filtrates were used to transmit the leukemia.
- Control specimens from non-leukemic mice were analyzed for comparison.
Main Results:
- Virus-like particles were detected in 12 out of 47 examined leukemia specimens.
- These particles were located within the cytoplasm of leukemic cells.
- Particles were approximately 78 nm in diameter and not found in control animals.
Conclusions:
- The observed particles resemble those found in other mouse tumors.
- The findings suggest a potential viral etiology for this type of mouse leukemia.
- Further research is warranted to confirm the role of these particles in leukemia development.
