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Updated: Sep 6, 2026

Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques
10:52

Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques

Published on: June 1, 2016

Light and electron microscope studies of mycobacterium--mycobacteriophage interactions. III. Further studies on the

K TAKEYA, M KOIKE, R MORI

    The Journal of Biophysical and Biochemical Cytology
    |November 1, 1961
    PubMed

    Insights

    Researchers studied mycobacteriophage B-1 multiplication using ultrathin sectioning. New phage particles emerged near the host cell

    Area of Science:

    • Microbiology
    • Virology
    • Cell Biology

    Background:

    • Mycobacteriophages are viruses that infect mycobacteria.
    • Understanding viral replication is crucial for controlling mycobacterial infections.

    Purpose of the Study:

    • To investigate the replication process of mycobacteriophage B-1 within its host cell.
    • To characterize the morphological changes during phage multiplication.

    Main Methods:

    • Utilized an improved ultrathin sectioning technique for electron microscopy.
    • Observed host cell and phage particle morphology throughout the infection cycle.

    Main Results:

    • The host cell's nuclear apparatus remained unchanged during the latent period.
    • Phage-shaped particles appeared approximately 30 minutes post-infection in specific cellular regions.
    • Mature and empty phage heads were observed at the end of the latent period.

    Conclusions:

    • Mycobacteriophage B-1 replication involves the formation of new particles in distinct areas of the host cell.
    • Empty phage head structures may be present within the host cell during the latent period.
    Keywords:
    BACTERIOPHAGEMYCOBACTERIUM

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