Related Experiment Videos
Determination of ascorbic acid in pharmaceuticals and urine by reverse flow injection
M I Albero1, M Soledad García, C Sánchez-Pedreño
1Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
The Analyst
|October 1, 1992
Summary
Two new flow injection (FI) methods accurately determine ascorbic acid (vitamin C) in pharmaceuticals and urine. These techniques utilize spectrophotometric detection for reliable quantification in various samples.
Area of Science:
- Analytical Chemistry
- Spectrophotometry
- Chemical Analysis
Background:
- Ascorbic acid (vitamin C) is a vital nutrient with significant implications in human health and pharmaceutical applications.
- Accurate and efficient determination of ascorbic acid is crucial for quality control in pharmaceuticals and for clinical diagnostics.
- Existing analytical methods may require improvements in sensitivity, speed, or applicability to complex matrices.
Purpose of the Study:
- To develop and validate two novel reverse flow injection (FI) methods for the spectrophotometric determination of ascorbic acid.
- To compare the performance characteristics, including working ranges, of the peak-height and peak-width FI techniques.
- To assess the applicability of the developed FI methods for quantifying ascorbic acid in real-world samples, specifically pharmaceuticals and urine.
Main Methods:
- Development of two distinct reverse flow injection analysis (FIA) systems utilizing spectrophotometric detection.
- Employing the reaction between ascorbic acid and ethylenediaminetetraacetic acid-CoIII complex in a 5% diethylamine medium.
- Utilizing peak-height and peak-width techniques for signal measurement in the respective FI methods.
Main Results:
- The peak-height FI technique demonstrated effective determination of ascorbic acid within the concentration range of 2 x 10(-4) to 5 x 10(-3) mol dm(-3).
- The peak-width FI method expanded the working range for ascorbic acid determination to 2 x 10(-3) to 5 x 10(-2) mol dm(-3).
- Both developed FI methods were successfully applied to the analysis of ascorbic acid in pharmaceutical formulations, with the peak-height method also validated for urine samples.
Conclusions:
- Two sensitive and efficient reverse flow injection spectrophotometric methods for ascorbic acid determination have been successfully developed.
- The peak-height and peak-width FI techniques offer complementary working ranges, enhancing the versatility of ascorbic acid analysis.
- These methods provide reliable quantification of ascorbic acid in pharmaceutical products and biological fluids like urine, demonstrating practical utility.