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Published on: November 28, 2019
Electron microscopy of Neurospora crassa mycelia
Abstract:
The fine structure of vegetative mycelia of the filamentous Ascomycete, Neurospora crassa, has been investigated by the standard techniques of electron microscopy. Addition of uranyl nitrate to the methacrylate-embedding medium minimized disruption of the specimens-an accident often observed in the preparation of microbial material. This report describes the presence of a chitinous polysaccharide wall containing fine fibrils embedded in a homogeneous matrix. A sinuate plasma membrane lies adjacent to the inner wall surface. This membrane is often closely associated with the endoplasmic reticulum of the cytoplasm. Numerous mitochondria of the classical type, and dense particles of 10 mmicro diameter occur throughout the cytoplasm. The nuclear region is surrounded by a double membrane with pore openings. Associated with the nuclear envelope is a dense area, the nucleolus. The significance of these observations and their relationship to other forms is discussed.
Insights
This study details the ultrastructure of Neurospora crassa mycelia using electron microscopy. Key findings include the chitinous cell wall, sinuate plasma membrane, and distinct nuclear structures.
Area of Science:
- Mycology
- Cell Biology
- Microscopy
Background:
- Understanding the cellular structure of filamentous fungi like Neurospora crassa is crucial for mycology.
- Previous studies often faced specimen disruption during preparation for electron microscopy.
Purpose of the Study:
- To investigate the fine structure of vegetative mycelia in Neurospora crassa.
- To describe the ultrastructural components of this filamentous Ascomycete.
Main Methods:
- Standard electron microscopy techniques were employed.
- Uranyl nitrate was added to the methacrylate-embedding medium to minimize specimen disruption.
Main Results:
- A chitinous polysaccharide cell wall with fibrils in a homogeneous matrix was observed.
- A sinuate plasma membrane, often associated with the endoplasmic reticulum, was identified.
- Numerous mitochondria, dense cytoplasmic particles, and a double-membraned nucleus with pores and a nucleolus were described.
Conclusions:
- The study provides a detailed ultrastructural description of Neurospora crassa vegetative mycelia.
- The findings offer insights into the cellular organization and potential functional relationships of organelles within this fungus.
