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[The dosage of SDS when extracting membranous labyrinth proteins]
Xiang Huang1, Xi Lin, Qing Cheng
1Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
Objective:
To determine the appropriate dosage of SDS when extracting membranous labyrinth proteins.
Method:
The yield of proteins extracted with various amounts of SDS were determined by BCA method, and the components of the extracted proteins were analyzed by SDS-PAGE.
Result:
The amount of proteins extracted with 0.1% SDS is less than that with 0.23%, 1% or 2% SDS, meanwhile it shows less bands than the other and the corresponding bands are stained weaker. 0.23% SDS is less efficiently than 1% and 2% SDS in solubilizing proteins. There is no significant difference between 2% and 1% SDS in yield of proteins, while 2% SDS accompanies a higher ratio of detergent to protein.
Conclusion:
1% SDS is solutions is relatively suitable for extracting membranous labyrinth proteins extensively while the ratio of detergent to protein is from 5 to 6.