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Different polymerase chain reaction-based analyses for culture-negative endocarditis caused by Streptococcus
Marianne Voldstedlund1, Lisbeth Nørum Pedersen, Kurt Fuursted
1Department of Clinical Microbiology, Skejby Hospital, Brendstrupgaardsvej, Aarhus, Denmark. mvold@dadlnet.dk
Scandinavian Journal of Infectious Diseases
|November 11, 2003
Abstract:
Two cases of culture-negative infective endocarditis caused by Streptococcus pneumoniae are presented. Conventional bacteriological methods were compared with 2 different polymerase chain reaction (PCR)-based analyses: PCR amplification with primers specific for S. pneumoniae, and broad-range PCR followed by sequencing. PCR-based analyses can be a valuable supplement to traditional bacteriological analyses.