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Related Experiment Videos

Analysis of parasites by electrorotation.

C Dalton1, A D Goater, J P H Burt

  • 1Institute of Bioelectronic and Molecular Microsystems, University of Wales, Bangor, Gwynedd, UK. colin@informatics.bangor.ac.uk

Journal of Applied Microbiology
|December 18, 2003
PubMed
Summary

Electrorotation, a noninvasive AC electrokinetic technique, effectively determines the viability and developmental stages of parasitic protozoa and nematodes. This versatile method aids in analyzing Cryptosporidium, Giardia, Cyclospora, and Ascaris transmission stages.

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Area of Science:

  • Parasitology
  • Biophysics
  • Microfluidics

Background:

  • Eukaryotic parasite transmission stages require accurate analysis for public health.
  • Electrorotation is a noninvasive AC electrokinetic technique using microelectrodes to study microorganisms.
  • This method probes the physiological structure of micro-organisms within microfluidic chambers.

Purpose of the Study:

  • To review the application of electrorotation for studying eukaryotic parasite transmission stages.
  • To describe the application of electrorotation to Cryptosporidium, Giardia, Cyclospora, and Ascaris.
  • To illustrate the effectiveness and versatility of electrorotation in parasitology.

Main Methods:

  • Utilized standard electrorotation apparatus including micro-fabricated electrodes, a fluidic chip, signal generator, microscope, and image capture.

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  • Recorded spectra of cellular rotation rate as a function of applied electric field frequency.
  • Compared results with standardized biological tests where applicable.

    • Main Results:

    • Achieved electrorotational determination of viability for Giardia intestinalis cysts, Cryptosporidium parvum oocysts, and Cyclospora cayetanensis oocysts.
    • Successfully determined the sporulation state of Cyclospora cayetanensis oocysts.
    • Determined the fertilization state of Ascaris suum ova.

    Conclusions:

    • Electrorotation is a simple, noninvasive, and versatile analytical technique.
    • The technique is suitable for a wide range of particle types.
    • Electrorotation is capable of incorporation into integrated Lab-on-a-chip devices for parasite analysis.