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Related Experiment Videos

Radiative decay engineering 3. Surface plasmon-coupled directional emission.

Joseph R Lakowicz1

  • 1Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA. lakowicz@cfs.umbi.umd.edu

Analytical Biochemistry
|December 24, 2003
PubMed
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A novel fluorescence detection method enhances sensitivity 20- to 1000-fold by coupling fluorophores with surface plasmons. This surface plasmon-coupled emission (SPCE) technique reduces autofluorescence and improves signal collection for bioscience applications.

Area of Science:

  • Optics and Photonics
  • Biophysics
  • Materials Science

Background:

  • Standard fluorescence detection methods suffer from limited sensitivity and interference from sample autofluorescence.
  • Improving signal-to-noise ratio is crucial for sensitive detection in biological and diagnostic applications.

Purpose of the Study:

  • To introduce and characterize a new fluorescence detection method based on surface plasmon-coupled emission (SPCE).
  • To demonstrate the potential of SPCE for significantly enhancing fluorescence detection sensitivity and reducing autofluorescence.

Main Methods:

  • Coupling excited fluorophores with surface plasmons on thin metal films (silver, gold).
  • Utilizing the phenomenon of surface plasmon-coupled emission (SPCE) for fluorophore detection within 20-250 nm of the metal surface.

Related Experiment Videos

  • Exploiting the narrow angular distribution and polarization of SPCE for efficient signal collection and autofluorescence reduction.

    • Main Results:

    • Achieved a 20- to 1000-fold increase in fluorescence detection sensitivity.
    • Significantly decreased the contribution of sample autofluorescence to the detected signal.
    • Demonstrated collection of up to 50% of emission, a substantial improvement over conventional methods.

    Conclusions:

    • SPCE offers a powerful new modality for highly sensitive fluorescence detection.
    • The method is versatile and applicable to various biosensing, diagnostic, and biotechnology applications.
    • SPCE is expected to have broad impact across the biosciences due to its enhanced performance and reduced interference.