The development of variable MLM editor and TSQL translator based on Arden Syntax in Taiwan

Yan Ching Liang1, Polun Chang

  • 1Graduate Institute of Health Informatics and Decision Making, National Yang-Ming University, Taiwan.

Related Concept Videos

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids07:14

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids

Here, we present a gene therapy approach that delivers ABE-coated chitosan directly to the bone marrow by intraosseous...
633
Analysis of Translation in the Developing Mouse Brain using Polysome Profiling08:38

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

The development of the mammalian brain requires proper control of gene expression at the level of translation. Here, we describe a polysome profiling system with an easy-to-assemble sucrose gradient-making and fractionation platform to assess the translational status of mRNAs in the developing...
5.6K
Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

People with BRCA1 mutations have a higher risk of developing cancer, which warrants accurate evaluation of the function of BRCA1 variants. Herein, we described a protocol for functional assessment of BRCA1 variants using CRISPR-mediated cytosine base editors that enable targeted C:G to T:A conversion in living...
5.9K
Standard Operating Procedure for Lyssavirus Surveillance of the Bat Population in Taiwan07:55

Standard Operating Procedure for Lyssavirus Surveillance of the Bat Population in Taiwan

This protocol introduces a standard laboratory operating procedure for diagnostic testing of lyssavirus antigens in bats in...
7.9K
Translation01:31

Translation

Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...
155.6K
Initiation of Translation02:33

Initiation of Translation

Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
38.4K