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Monitoring eukaryotic gene expression using oligonucleotide microarrays.

Jennifer Lescallett1, Marina E Chicurel, Robert Lipshutz

  • 1Affymetrix Inc., Santa Clara, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 19, 2004
PubMed
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This chapter details using oligonucleotide microarrays, specifically GeneChip arrays, for global gene expression profiling. It covers protocols from target preparation to data analysis and sharing for biological and medical research.

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Global gene expression analysis is crucial for modern biological and medical research.
  • Oligonucleotide microarrays offer a powerful tool for achieving comprehensive gene expression views.

Purpose of the Study:

  • To describe the application of oligonucleotide microarrays, focusing on GeneChip arrays, for global gene expression analysis.
  • To provide a detailed guide on protocols, including target preparation, hybridization, signal detection, and data analysis.
  • To discuss strategies for data sharing and integration with existing genomic databases.

Main Methods:

  • Utilizing GeneChip arrays for high-throughput gene expression profiling.
  • Detailed description of experimental protocols: target preparation, hybridization, washing, and signal detection.

Related Experiment Videos

  • Explanation of data analysis techniques and methods for facilitating data sharing.

    • Main Results:

    • GeneChip arrays demonstrate high reproducibility, sensitivity, and specificity for gene expression analysis.
    • Comprehensive protocols are provided for researchers to perform genomic profiling.
    • Methods for data sharing and contextualization within public and proprietary databases are discussed.

    Conclusions:

    • Oligonucleotide microarrays, particularly GeneChip arrays, are essential tools for global gene expression studies.
    • The chapter serves as a guide to genomic profiling, emphasizing current protocols and data management.
    • Researchers are encouraged to consult updated resources for the latest advancements in microarray technology.