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Circadian variations of bone marrow engraftability
Lionel D'Hondt1, Christina McAuliffe, Jeffrey Damon
1Centre Hospitalier Notre-Dame et Reine Fabiola, Charleroi, Belgium.
Journal of Cellular Physiology
|May 12, 2004
Summary
Circadian rhythms impact bone marrow engraftment. Bone marrow transplant strategies should consider these daily variations in donor cells for improved outcomes.
Area of Science:
- Hematology
- Chronobiology
- Immunology
Background:
- Circadian rhythms influence physiological processes, including hematopoiesis.
- Limited knowledge exists regarding the daily variation in bone marrow engraftability and host acceptance.
Purpose of the Study:
- To investigate the impact of circadian variations on bone marrow engraftability.
- To determine if host acceptance of bone marrow grafts is affected by circadian rhythms.
Main Methods:
- Utilized a congenic mouse model (B6.SJL to C57BL/6J).
- Assessed bone marrow engraftment at various Hours After Light Onset (HALO) points (4, 8, 12, 16, 20, 24).
- Analyzed engraftment in bone marrow, spleen, and thymus, and progenitor cell cycle status.
Main Results:
- Significant variations in bone marrow engraftability were observed across different time points.
- Two significant nadirs in engraftment occurred at HALO 8 and 24 in July experiments.
- Marrow progenitor cell proliferation peaked at HALO 8 and 24.
- Host graft acceptance remained unaffected by host circadian cycle variations.
Conclusions:
- Bone marrow engraftability exhibits significant circadian variation.
- These daily rhythms in donor cells are crucial and must be considered in bone marrow transplant protocols.
- Host immune acceptance is not influenced by circadian variations in the context of this study.