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Updated: Aug 24, 2026

A Continuous Culture Millifluidic Device for the Study of Escherichia coli under Low and Fluctuating Nutrient Conditions
Published on: July 7, 2026
High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-stat
Beom Soo Kim1, Seung Chul Lee, Sang Yup Lee
1Department of Chemical Engineering, Chungbuk National University, Cheongju, Korea. bskim@chungbuk.ac.kr
Abstract:
A new feeding strategy in fed-batch culture, exponential feeding combined with pH-stat is suggested to avoid the accumulation of substrate in culture broth. Exponential feeding was stopped whenever a predetermined amount of limiting substrate was supplied and then pH change was observed. When pH rose above an upper limit due to the depletion of substrate, feeding was restarted. With this feeding strategy, recombinant Escherichia coli could be grown to 101 g/l by controlling the specific growth rate at 0.1 h(-1).
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