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Updated: Aug 24, 2026
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
Published on: January 22, 2014
[Construction, expression and biologic activities of two rhIL-6/GM-CSF fusion proteins]
Qiang-ming Sun1, Mao-sheng Sun, Shan Yi
1Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming 650118, China. sunqiangming1@sina.com
Aim:
To construct and express the gene encoding hIL-6/GM-CSF fusion protein.
Methods:
The genes encoding the two hIL-6/GM-CSF fusion proteins were constructed in pBV220 expression vector. Fusion proteins were expressed in E.coli BL-21 and purified through Q Sepharose HP ion exchange chromatography and Sephacryl S-200 gel filtration columns. The biologic activities of the fusion proteins were detected by proliferation of hIL-6 dependent cell line B9 and hGM-CSF dependent cell line TF1 with MTT assay.
Results:
Both fusion proteins were expressed in E.coli BL-21 in the form of inclusion body. The expression levels were more than 25% of the total cell lysates. Both fusion proteins were obtained with high purity which had both hIL-6 and hGM-CSF biologic activities.
Conclusion:
Two hIL-6/GM-CSF fusion proteins with high purity and bilolgic activities have been acquired successfully.
