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Published on: October 15, 2019
Potassium hexacyanoferrate(III)-mediated fluorometric determination of melatonin
Hideaki Iizuka1, Takehiko Yajima
1Faculty of Pharmaceutical Sciences, Toho University 2-2-1 Miyama Funabashi, Chiba 274-8510, Japan. iizuka@phar.toho-u.ac.jp
Abstract:
This paper describes the development of a novel, simple and sensitive high-performance liquid chromatographic method for the determination of melatonin using precolumn fluorometric derivatization. A strong fluorescence was induced when alkaline solution of melatonin was heated in the presence of potassium hexacyanoferrate (III). This fluorescent reaction was used for the precolumn derivatization of melatonin. Solid phase extraction was used for concentrating melatonin below a level of detection limit.
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