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[Application of hard tissue slicing in research of bone tissue engineering]
Xiuqun Li1, Li Deng, Jingcong Luo
1Division of Stem Cell and Tissue Engineering, Key Laboratory of Biotherapy of Human Diseases Ministry of Education, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, P. R. China.
Objective:
To improve the method of hard tissue slicing for research of bone tissue engineering.
Methods:
The method of hard tissue slicing especially the key technique-slice mounting had been studied and improved.
Results:
The experiments showed that our research improved techniques in hard tissue slicing not only in keeping the characteristics of hard tissue slicing, easily stain with HE, Masson, immunohistochemistry, and distinguishing the differences among tissues easily, but also in overcoming the shortcoming of issue shedding easy to happen in the general hard tissue slicing.
Conclusion:
The improved techniques in hard tissue slicing is a more useful hard tissue slicing techniques.
