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Analysis of protein-protein proximities using the split-ubiquitin system
1Department of Microbiology, Faculty of Medicine, National University of Singapore. micln@nus.edu.sg
Briefings in Functional Genomics & Proteomics
|July 9, 2004
Abstract:
The split-ubiquitin system is a fragment complementation assay that is based on a conditional proteolysis design. The system has been used to study protein-protein interactions between membrane proteins and to screen for new interaction partners for transcription factors. This paper outlines the recent progress in the split-ubiquitin technology that has been made possible through the development of new reporter proteins.