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Updated: Aug 23, 2026

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
Published on: May 22, 2013
Real-time quantitative assay of telomerase activity using the duplex scorpion primer
Yanping Huang1, Deming Kong, Yi Yang
1State Key Lab of Functional Polymer Materials for Adsorption and Separation, Chemical School, Nankai University, Tianjin 300071, PR China.
Abstract:
A novel real-time quantitative method for measuring telomerase activity is described in which the duplex scorpion primer is used to provide an intramolecular probing mechanism for specific detection of telomerase activity. Using this method, linearity from 10 to 10(4) cells expressing telomerase activity could be obtained (R2 = 0.994). The requirement of post-PCR steps is thus obviated.
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