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Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
Published on: May 24, 2017
Atomic force microscopy studies on DNA structural changes induced by vincristine sulfate and aspirin
Yi Zhu1, Hu Zeng, Jianming Xie
1Laboratory of Molecular and Biomolecular Electronics, Southeast University, Nanjing 210096, P.R. China.
Abstract:
We report that atomic force microscopy (AFM) studies on structural variations of a linear plasmid DNA interact with various concentrations of vincristine sulfate and aspirin. The different binding images show that vincrinstine sulfate binding DNA chains caused some loops and cleavages of the DNA fragments, whereas aspirin interaction caused the width changes and conformational transition of the DNA fragments. Two different DNA structural alternations could be explained by the different mechanisms of the interactions with these two components. Our work indicates that the AFM is a powerful tool in studying the interaction between DNA and small molecules.
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