Related Experiment Video
Updated: Aug 22, 2026
SCAnED - An Open-source Skin Segmentation Macro for Semi-automated Cell and Nuclei Detection in Epidermal and Dermal Skin Compartments
Published on: August 8, 2025
Use of digital epiluminescence microscopy to help define the edge of lentigo maligna
1Division of Dermatology, Department of Medicine, Cardinal Bernardin Cancer Center, Loyola University Stritch School of Medicine, Maywood, IL 60153, USA. jrobin5@lumc.edu
Objective:
To compare identification of the border of lentigo maligna (LM) with digital epiluminescence microscopy (DELM) with clinical and Wood light assessment.
Design:
The borders of lesions identified clinically with the Wood light, with DELM, and after excision by Mohs micrographic surgery were traced onto plastic sheets. The borders defined on the tracings were compared for congruence and mean surface area.
Setting:
Cardinal Bernardin Cancer Center for Skin Cancer, Loyola University Health System, Maywood, Ill.
Patients:
Twenty-six consecutive patients with LM of the head and neck.
Main Outcome Measures:
Results of the comparison of the outlines of the borders and the mean surface area identified by the 4 methods.
Results:
The border determined by clinical examination was smaller than that determined with the Wood lamp or by DELM. Most lesions underwent an additional excision 5 mm beyond the DELM-defined border. The DELM pattern of LM with asymmetric follicular openings and dark brown rhomboidal structures changed at the periphery and became a pigmented thin mesh that was associated with the histopathological features of melanoma in situ. More homogeneous pigmented areas extending from the LM were associated with the pathologic features of melanocytic hyperplasia.
Conclusions:
Visualization of LM by DELM (dermoscopy) helps to guide resection. Because LM arises in sun-damaged skin with melanocytic hyperplasia, determining the tumor-free margin requires the judgment of an experienced physician.
