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Updated: Aug 20, 2026

Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats
Published on: May 24, 2018
[The expression pattern of L-type voltage-gated Ca2+ channel in rat cochlea]
Weidong Shen1, Yinyan Hu, Weiyan Yang
1Department of Otolaryngology-Head and Neck Surgery, PLA General Hospital, Beijing, 100853, China.
Objective:
To investigate the molecular basis of the voltage-gated Ca2+ channel (VGCC) which controls the afferent synaptic neurotransmitters release in inner hair cell and their cellular expression.
Method:
In this study we used different and complementary approaches to determine which types of 4 subunits of VGCC were expressed in the organ of Corti.
Result:
RT-PCR analysis showed that alpha1C and alpha1D were present in the organ of Corti total RNA extract, and the corresponding antisense riboprobes could detect the expression of alpha1C and alpha1D in IHCs and OHCs.
Conclusion:
The voltage-gated Ca2+ channel expressed in sensory cell in organ of Corti are alpha1C and alpha1D subtypes.
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