[Determination of synephrine and N-methyltyramine in Pericarpium Citri Reticulatae viride by HPLC]
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Published on: March 1, 2018
Xian-duan Li1, Zhi-jing Ma, Sheng Lin
1Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Objective:
To establish a quantitative method for determination of synephrine and N-methyltyramine in Citri Reticulatae.
Method:
Samples were extracted with 30% methanol. ODS column was used with methanol-water-sodium dodecyl sulfate (55:45:0.1) as mobile phase. Detection wavelength was 285 nm.
Result:
Synephrine and N-methyltyramine in sample solution were well separated. Linearity of synephrine was good (r = 0.9999) in range of 0.35-11.24 microg. The average recovery was 97.1%, and RSD of repeatability was 1.9%.
Conclusion:
This method can be used for quality control of Citri Reticulatae.
