[Analysis of PTH amino acids by micellar electrokinetic capillary chromatography]

A useful method for separation of 19 PTH amino acids by micellar electrokinetic capillary chromatography has been developed. Experiments were performed at 35 degrees C with 14kV power, 50microm x 57cm fused-silica capillary column and UV absorption detector. Each new capillary was washed with 0.1mol/L NaOH and deionized water, each for 15min, before use. Samples were injected into the capillary by applying pressure (3402Pa) for 5s. Absorption detection was at 200nm. The running buffer was made up of 50mmol/L sodium dodecyl sulfate (SDS), 50mmol/L sodium of phosphate and borate (pH 7. 0). The infulence of operation voltage, pH of buffer, temperature and SDS concentration on the amino acids migration has been studied. The coefficients of variaton of migration time and peak area of 19 PTH amino acids were below 1.0% and 5.0% respectively. The detection limits were 0.1-0.25pmol.