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Updated: Aug 19, 2026
Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
Published on: October 15, 2016
[Screening of human scFv against IL-8 from phage antibody library]
Lei Wang1, Yuan-yuan Qiao, Gang Wang
1Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Aim:
To screen human scFv against IL-8 from phage antibody library.
Methods:
IL-8-His fusion protein was expressed in E.coli BL21 (DE3) transfected with prokaryotic expression vector pRSET-IL-8 and was purified by affinity chromatography. Specific antibody was screened by 3 rounds of panning of phage antibody library with the fusion protein. The antigen binding activity and DNA sequences of positive clones were determined and analyzed.
Results:
After 3 rounds of panning, 2 positive clones were obtained which could bind IL-8 specifically. DNA sequence analysis showed both of the 2 VH genes belonged to human IgG VH3 subgroup, and the Vlambda gene belonged to human VlambdaDPL5 and VlambdaDPL2 subgroups,respectively.
Conclusion:
It is feasible to obtain human anti-IL-8 antibody by phage display technique, which provides the basis for further research on psoriasis and other related diseases.
