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Updated: Aug 13, 2026
Induction and Testing of Hypoxia in Cell Culture
Published on: August 12, 2011
[Expression of hypoxia-inducible factor-1alpha in middle ear cholesteatoma]
Yang Liu1, Yonghua Cui, Linlin Yu
1Department of Otolaryngology, Tongji Hospital, Wuhan, 430030, China. yangliu616@yahoo.com.cn
Objective:
To explore the pathogenetic mechanism of middle ear cholesteatoma, the aim of this study is to detect the expression of Hypoxia-inducible factor-1alpha in middle ear cholesteatoma and normal external ear canal skin.
Method:
We used the technology of immunohistochemistry to examine the expression of Hypoxia-inducible factor-1alpha in thirty-one middle ear cholesteatomas and ten samples of normal external ear canal skin.
Result:
The expression of HIF-1alpha was extremely higher in middle ear cholesteatomas than in normal external ear canal skin (P <0.05).
Conclusion:
We found that the higher expression of Hypoxia-inducible factor-1alpha in middle ear cholesteatomas, so we think that Hypoxia-inducible factor-1alpha play an important role in the pathogenetic process of middle ear cholesteatoma, and hypoxia may be an incentive in the pathogenetic mechanism of middle ear cholesteatoma.
