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Related Experiment Videos

Clinical experience with embryos produced by handmade cloning: work in progress.

Hanne G Pedersen1, Mette Schmidt, Per T Sangild

  • 1Department of Clinical Studies, Reproduction, Royal Veterinary and Agricultural University, Dyrlaegevej 68, DK-1870 Frederiksberg C, Denmark. hgp@kvl.dk

Molecular and Cellular Endocrinology
|April 20, 2005
PubMed
Summary

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Handmade cloning (HMC) in cattle shows promise, but results in premature calves with health issues like organ enlargement and metabolic problems. Further research is needed to improve outcomes for cloned cattle.

Area of Science:

  • Reproductive biology
  • Animal cloning
  • Veterinary medicine

Background:

  • Somatic cell nuclear transfer (SCNT) in cattle is associated with high rates of pregnancy loss and developmental abnormalities.
  • Existing cloning methods often lead to conceptus loss, hydrallantois, and neonatal mortality.

Purpose of the Study:

  • To evaluate the in vivo development of cattle embryos produced via a novel handmade cloning (HMC) technique.
  • To characterize the health and development of premature calves born from HMC pregnancies.

Main Methods:

  • Day 7 embryos (fresh and vitrified) from aggregated or single cells were transferred to synchronized Holstein-Friesian heifers.
  • Pregnancy rates were monitored at various stages (days 28, 42, 63, 250).
  • Premature calves delivered via Caesarian section were weighed, and their organs and physiological parameters were analyzed.

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Main Results:

  • Aggregated embryos showed higher developmental competence (67% pregnancy rate) compared to single embryos (38%).
  • Overall delivery rate was 9%, with one case of hydrallantois.
  • Premature calves exhibited increased body and organ weights, oversized placentomes, and metabolic and respiratory dysfunction.

Conclusions:

  • Handmade somatic cell cloning (HMC) in cattle results in a low delivery rate and significant fetal and neonatal abnormalities.
  • Cloned calves displayed disproportionate organ growth, metabolic immaturity, and respiratory compromise.
  • Further optimization of HMC is required to mitigate adverse outcomes in cloned cattle.