Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Approaches for coupling solid-phase microextraction to nanospray.

Markus Walles1, Yu Gu, Catherine Dartiguenave

  • 1Department of Chemistry, University of Waterloo, Waterloo, Ont, Canada N2L 3G.

Journal of Chromatography. A
|April 23, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Universal carbon nanotubes-polybenzimidazole SPME coating and its application for both gas and liquid chromatography.

The Analyst·2024
Same author

Deactivated fused silica tubing: A solution for flexible time weighted averaging needle trap sampling.

Analytica chimica acta·2024
Same author

Upregulation of ALOX12-12-HETE pathway impairs AMPK-dependent modulation of vascular metabolism in ApoE/LDLR<sup>-/-</sup> mice.

Pharmacological research·2024
Same author

3D Printed Coated Blade Spray-Mass Spectrometry Devices.

Analytical chemistry·2024
Same author

Identification of Omitted Pollutants in Environmental Water via In Situ Thin-Film Microextraction.

Analytical chemistry·2024
Same author

Perspective on <i>in vivo</i> SPME for human applications: Starting from monitoring doxorubicin during lung chemo-perfusion.

Journal of pharmaceutical analysis·2024
Same journal

Retention prediction in reversed-phase liquid chromatography using XGBoost-based quantitative structure-retention relationships models.

Journal of chromatography. A·2026
Same journal

Impurity profiling of lipid-conjugated oligonucleotides using reversed-phase with and without ion-pair reagents and hydrophilic interaction liquid chromatography.

Journal of chromatography. A·2026
Same journal

Preparation of magnetic zwitterionic covalent organic frameworks for rapid simultaneous extraction of hydrophilic and hydrophobic organophosphates from environmental waters coupled with UHPLC-MS/MS determination.

Journal of chromatography. A·2026
Same journal

Analysis of organic and inorganic acids in biomass pyrolysis process samples by ion chromatography-mass spectrometry.

Journal of chromatography. A·2026
Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026
Same journal

Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process intensification.

Journal of chromatography. A·2026
See all related articles

New biocompatible solid-phase microextraction (SPME) devices using restricted access materials (RAM) enable direct coupling to mass spectrometry for peptide analysis. This method simplifies sample preparation and reduces analysis time for tryptic digests.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Solid-phase microextraction (SPME) is a widely used technique for sample preparation.
  • Restricted access materials (RAM) offer selectivity by excluding large molecules.
  • Direct coupling of sample preparation to mass spectrometry (MS) can streamline analytical workflows.

Purpose of the Study:

  • To develop and evaluate biocompatible SPME devices coated with RAM for peptide analysis.
  • To investigate the direct coupling of RAM-SPME to nanospray mass spectrometry (MS).
  • To assess the efficiency of in situ peptide extraction from tryptic digests.

Main Methods:

  • Preparation of SPME devices using RAM immobilized on steel and platinum wires.
  • Interfacing SPME devices to MS via electrospray ionization (ESI).

Related Experiment Videos

  • Immobilization of trypsin for in situ protein digestion and subsequent peptide extraction and analysis by SPME/nanospray.

    • Main Results:

    • RAM-SPME coatings effectively reduced matrix interference, enabling direct MS coupling without further purification.
    • The vial approach for in situ digestion and extraction yielded optimal results, identifying up to eight peptides with 58% sequence coverage.
    • The limit of detection for SPME/nanospray was approximately 50 fmol/mL for peptides in aqueous solution.

    Conclusions:

    • Direct coupling of RAM-SPME to nanospray MS is a viable and efficient method for peptide analysis.
    • This approach offers reduced analysis time and simplifies workflows compared to conventional methods like Zip-Tip purification.
    • The developed SPME devices demonstrate potential for high-throughput proteomic analyses.