Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Growing E. coli to high cell density--a historical perspective on method development.

Joseph Shiloach1, Rephael Fass

  • 1Biotechnology Unit, Bldg. 14A Rm. 173, NIDDK, NIH Bethesda, MD 20892-5522, USA. yossi@nih.gov

Biotechnology Advances
|May 19, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome-Wide High-Throughput RNAi Screening for Identification of Genes Involved in Protein Production.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Aotus nancymaae model predicts human immune response to the placental malaria vaccine candidate VAR2CSA.

Lab animal·2023
Same author

Coordinated expression of acetyl CoA synthetase and the <i>ace</i> operon enzymes in <i>Escherichia coli</i> in preparation for adaptation to acetate.

Microbiology (Reading, England)·2022
Same author

Iron availability enhances the cellular energetics of aerobic Escherichia coli cultures while upregulating anaerobic respiratory chains.

New biotechnology·2022
Same author

Expression and production of pigment epithelium-derived factor (PEDF) and PEDF receptor variants from mammalian and bacterial cells.

Protein expression and purification·2022
Same author

Affecting HEK293 Cell Growth and Production Performance by Modifying the Expression of Specific Genes.

Cells·2021
Same journal

Cell surface display for nutritional chemicals: Strategies, mechanisms, and evaluation methods.

Biotechnology advances·2026
Same journal

Advancing synthetic biology with engineered chemically inducible gene regulatory systems.

Biotechnology advances·2026
Same journal

Technology-driven revolution in CO<sub>2</sub> fixation: From natural pathways to programmable Biosystems.

Biotechnology advances·2026
Same journal

Enzymes for CO<sub>2</sub> fixation: Discovery, engineering, and applications.

Biotechnology advances·2026
Same journal

Technological advances in extrachromosomal circular DNA detection.

Biotechnology advances·2026
Same journal

Codon compression and novel codon creation for multiplex non-canonical amino acid incorporation.

Biotechnology advances·2026
See all related articles

High-density bacterial cultures of Escherichia coli (E. coli) are crucial for producing heterologous proteins. Advanced fermentation techniques and optimized conditions enable E. coli to reach high cell densities, maximizing productivity.

Area of Science:

  • Microbiology
  • Biotechnology
  • Bioprocess Engineering

Background:

  • Escherichia coli (E. coli) is a primary bacterial host for recombinant protein production.
  • Achieving high cell densities in E. coli cultures is essential for maximizing productivity.
  • Research since the 1970s has focused on overcoming limitations in bacterial culture density.

Purpose of the Study:

  • To review strategies for achieving high-density E. coli cultures for protein expression.
  • To identify key factors influencing bacterial growth and productivity.
  • To suggest future research directions for enhancing E. coli's protein production capabilities.

Main Methods:

  • Development of batch, fed-batch, and dialysis fermentation techniques.
  • Optimization of media composition for high-density growth.

Related Experiment Videos

  • Application of molecular biology methods to enhance E. coli physiology.

    • Main Results:

    • E. coli can be cultivated to cell densities of up to 190 g/l (dry weight).
    • Methods were developed to prevent media precipitation and acetate accumulation.
    • Established techniques allow for high-yield production of heterologous proteins.

    Conclusions:

    • High-density cultivation of E. coli is achievable through optimized fermentation and media strategies.
    • Further research into heterologous protein biosynthesis effects can enhance E. coli productivity.
    • E. coli remains a vital platform for industrial-scale protein production.