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Plasma creatinine and creatine quantification by capillary electrophoresis diode array detector
Angelo Zinellu1, Marcello A Caria, Claudio Tavera
1Chair of Clinical Biochemistry, University of Sassari, Viale San Pietro 43/B, I-07100 Sassari, Italy.
Analytical Biochemistry
|June 2, 2005
Summary
This study presents a new capillary electrophoresis method for simultaneously measuring creatine and creatinine in human plasma. This technique offers a simpler and more efficient approach compared to traditional assays.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Traditional assays for nonprotein nitrogen compounds like creatine and creatinine often require separate measurements and are difficult to apply to plasma samples.
- Existing methods face challenges in simultaneous quantification and plasma applicability.
Purpose of the Study:
- To develop a simple, free zone capillary electrophoresis method for the simultaneous measurement of creatine and creatinine in human plasma.
- To optimize analytical parameters for enhanced resolution and efficiency.
Main Methods:
- Free zone capillary electrophoresis using an uncoated silica capillary.
- Optimization of Tris-phosphate running buffer (concentration, pH) and cartridge temperature.
- Method validation by comparison with a validated capillary electrophoresis assay and assessment in volunteers before/after exercise.
Main Results:
- Achieved good separation of creatine and creatinine in under 8 minutes using specific buffer conditions (75 mmol/L Tris-phosphate, pH 2.25) and temperature (15°C).
- Demonstrated method's performance through comparison with a validated assay and evaluation in volunteers undergoing physical exercise.
Conclusions:
- The developed capillary electrophoresis method provides a simple and efficient means for simultaneous creatine and creatinine measurement in human plasma.
- This method shows potential for clinical applications where simultaneous quantification is beneficial.