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[Phage typing and lysogen typing of Staphylococcus aureus]
Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
|November 1, 1979
Summary
Lysogenic typing is less consistent than phage typing for identifying Staphylococcus aureus strains. It is best used when phage typing with the International Basic Set fails, as restriction and modification drive lytic reaction specificity.
Area of Science:
- Microbiology
- Bacteriology
- Epidemiology
Background:
- Staphylococcus aureus (S. aureus) outbreaks necessitate reliable strain identification methods.
- Phage typing and lysogenic typing are established techniques for bacterial characterization.
- Understanding the specificity of lytic reactions is crucial for accurate typing.
Purpose of the Study:
- To compare the efficacy of phage typing and lysogenic typing for S. aureus.
- To evaluate the consistency of typing results from strains of the same origin over time.
- To investigate the role of restriction and modification in lytic reaction specificity.
Main Methods:
- Comparative analysis of phage typing and lysogenic typing results.
- Isolation and characterization of S. aureus strains from infection outbreaks and carriers.
- Experiments with induced phages in restriction-defective strains.
Main Results:
- Lysogenic typing exhibited greater variability in reaction numbers compared to phage typing for S. aureus strains.
- Lysogenic typing proved less reliable for routine identification of S. aureus.
- Restriction and modification were identified as key factors determining the specificity of phage-host interactions.
Conclusions:
- Phage typing is generally more reliable for S. aureus identification than lysogenic typing.
- Lysogenic typing is a supplementary method, useful only when standard phage typing is inconclusive.
- The specificity of lytic reactions is primarily governed by bacterial restriction-modification systems.