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Amitriptyline and nortriptyline serum determination by micellar liquid chromatography
Devasish Bose1, Abhilasha Durgbanshi, Adrià Martinavarro-Domínguez
1Area de Química Analítica, Universitat Jaume I, Campus Riu Sec, Av. de Vicent Sos Baynat, s/n, 12071 Castelló, Spain.
Journal of Pharmacological and Toxicological Methods
|July 28, 2005
Summary
A new micellar liquid chromatography method simplifies the measurement of amitriptyline and nortriptyline in serum for therapeutic drug monitoring. This technique offers a straightforward approach for routine clinical analysis.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Amitriptyline and nortriptyline are tricyclic antidepressants that enhance norepinephrine and serotonin activity.
- Their mechanism involves blocking neurotransmitter re-uptake at the neuronal membrane.
Purpose of the Study:
- To develop a micellar liquid chromatographic procedure for determining amitriptyline and nortriptyline in serum.
- To facilitate clinical monitoring of these antidepressant drugs.
Main Methods:
- Utilized a C18 column with a micellar mobile phase (0.15 M SDS-6% pentanol, pH 7).
- Employed electrochemical detection at 650 mV with a flow-rate of 1.5 mL/min.
- Analysis time was optimized to 14 minutes.
Main Results:
- Achieved limits of detection of 0.25 ng/mL for amitriptyline and 0.31 ng/mL for nortriptyline in serum.
- Demonstrated good repeatability and intermediate precision across various serum concentrations.
Conclusions:
- The developed method allows direct injection of filtered, untreated serum samples.
- This simple procedure is suitable for routine Therapeutic Drug Monitoring (TDM) applications.