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Resolving overlap in two-dimensional NMR spectra: nuclear Overhauser effects in a polysaccharide
Eriks Kupce1, Toshiaki Nishida, Göran Widmalm
1Varian Ltd, Yarnton, Oxford, UK.
Abstract:
Overlapping resonances in the two-dimensional nuclear Overhauser (NOESY) spectrum of the O-antigenic polysaccharide from Escherichia coli O147 have been resolved by recording a tilted projection of a three-dimensional NOESY-HSQC spectrum, where the carbon-13 and proton evolutions are linked together. Through the introduction of small contributions from the appropriate carbon-13 shifts, a cluster of five unresolved proton peaks is separated into its components without the need to perform the full three-dimensional measurement, a time saving of at least an order of magnitude.
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