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Surface plasmon resonance: applications in understanding receptor-ligand interaction
1Virology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002 India. ppat@rediffmail.com
Applied Biochemistry and Biotechnology
|August 25, 2005
Summary
Surface Plasmon Resonance (SPR) biosensors have advanced analytical techniques for studying receptor-ligand interactions. This review covers SPR technology and its diverse applications in biological research.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Biotechnology
Background:
- Significant advancements in analytical techniques for receptor-ligand interaction evaluation have occurred over the last decade.
- Surface Plasmon Resonance (SPR)-based optical biosensors are increasingly utilized for kinetic analysis of molecular interactions.
Purpose of the Study:
- To review Surface Plasmon Resonance (SPR) technology.
- To highlight key applications of SPR in understanding receptor-ligand interactions.
Main Methods:
- The review focuses on the principles and applications of SPR technology.
- It discusses the evolution of experimental design and data analysis methods in SPR.
Main Results:
- SPR biosensors enable the kinetic characterization of macromolecular and small molecule interactions.
- Applications span ligand fishing, microbiology, virology, host-pathogen interactions, and epitope mapping.
Conclusions:
- SPR technology has profoundly impacted basic and applied research in receptor-ligand interactions.
- It has led to significant changes in methodology, analysis, interpretation, and application within the field.