Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Arabitol dehydrogenase as a selectable marker for rice.

P R LaFayette1, P M Kane, B H Phan

  • 1Center for Applied Genetic Technologies, University of Georgia, 111 Riverbend Road, Athens, GA 30602-6810, USA.

Plant Cell Reports
|September 10, 2005
PubMed
Summary

Arabitol dehydrogenase enables efficient plant selection. This method uses arabitol to metabolize xylulose in plant cells, proving effective for rice transformation and selection.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Loss of flavonol 3-O-glucosyltransferase activity confers soybean resistance to leaf-chewing insects.

The Plant journal : for cell and molecular biology·2026
Same author

Advance care planning and health literacy in older dialysis patients: qualitative interview study.

BMJ supportive & palliative care·2021
Same author

Advance care planning in older dialysis patients: health care literacy qualitative study.

BMJ supportive & palliative care·2021
Same author

Advance care plan barriers in older patients with end-stage renal disease: a qualitative nephrologist interview study.

BMJ supportive & palliative care·2019
Same author

A protocol for repetitive somatic embryogenesis from mature peanut epicotyls.

Plant cell reports·2019
Same author

Growth characteristics and transformability of soybean embryogenic cultures.

Plant cell reports·2019

Area of Science:

  • Plant biotechnology
  • Molecular biology
  • Genetics

Background:

  • Traditional plant selectable markers can have limitations.
  • Arabitol dehydrogenase (atlD) offers a novel approach.
  • Arabitol is a sugar alcohol not typically metabolized by plants.

Purpose of the Study:

  • To evaluate arabitol dehydrogenase as a plant selectable marker.
  • To assess its efficiency in rice transformation.
  • To confirm gene integration and inheritance.

Main Methods:

  • Arabitol dehydrogenase gene (atlD) synthesized and optimized for plant expression.
  • Biolistic-mediated transformation used for rice.
  • Selection performed on arabitol and sucrose media.
  • Molecular analyses (gene integration and inheritance) conducted.

Related Experiment Videos

Main Results:

  • Transformation efficiency with arabitol (9.3%) was comparable to hygromycin (9.2%).
  • The atlD gene was successfully integrated into the rice genome.
  • Selected genes were inherited in a Mendelian fashion.

Conclusions:

  • Arabitol dehydrogenase is a viable and effective plant selectable marker.
  • This system offers an alternative selection strategy for plant biotechnology.
  • Further applications in crop improvement are possible.