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[Determination gentiopicrin in biyanqingdu granulae by SPE-HPLC]
1Guangdong Province Hospital of Troditional Chinese Medicine, Guangzhou.
Objective:
The purpose was to develop SPE-HPLC method for the assay of gentiopicrin in Biyanqingdu Granulae.
Methods:
Separation was performed on a hypersil ODS(2) column (250 mm x 4.6 mm, 5 microm). The mobile phase consisted of methanol: water (30:70, v/v). The flow rate was 1 ml/min. The UV detect wavelength was set at 275nm.
Results:
Gentiopicrin had a good linear relation in the range of 0. 02235 - 0. 1676 mg/ml, the average recovery was 98.1% with RSD 1.7%.
Conclusion:
The method is simple, sensitive and rapid and it can be applied to determine the content of Biyanqingdu Granulae.
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