Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spectral- and frequency-encoded fluorescence imaging.

Jason T Motz1, Dvir Yelin, Benjamin J Vakoc

  • 1Harvard Medical School and the Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, USA. jmotz@partners.org

Optics Letters
|October 29, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficient algorithm for optimal wavelength selection in photoacoustic spectral unmixing.

Biomedical optics express·2026
Same author

Polarization-sensitive optical coherence tomography-based fully-automated volumetric coronary fibrous cap characterization.

European heart journal. Imaging methods and practice·2026
Same author

Erratum: Wavenumber-space wavefront sensorless adaptive-optics for optical coherence tomography: publisher's note.

Biomedical optics express·2026
Same author

Wavenumber-space wavefront sensorless adaptive-optics for optical coherence tomography.

Biomedical optics express·2026
Same author

Development and characterization of a clinically viable metalens-enhanced optical endoscope.

Optics express·2025
Same author

Polarization-Sensitive Module for Optical Coherence Tomography Instruments.

ArXiv·2025
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

This study introduces a novel fluorescence imaging technique using spectral and frequency encoding to achieve high-resolution images. The method enables the development of advanced submillimeter endoscopes for detailed visualization.

Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Microscopy

Background:

  • Traditional fluorescence imaging methods face limitations in resolution and speed.
  • Developing advanced imaging techniques is crucial for minimally invasive diagnostics and research.

Purpose of the Study:

  • To present a new fluorescence imaging method utilizing spectral and frequency encoding.
  • To demonstrate the capability of achieving a high number of resolvable points in fluorescence images.

Main Methods:

  • Broadband excitation light is modulated with wavelength-dependent frequency.
  • A grating disperses light to illuminate an entire image line simultaneously.
  • Image lines are constructed using Fourier transform of frequency-encoded fluorescence and mechanical scanning.

Related Experiment Videos

Main Results:

  • The technique successfully generates high-resolution fluorescence images.
  • Validation was performed by imaging indocyanine green in microfluidic channels.
  • The method is suitable for creating submillimeter diameter endoscopes.

Conclusions:

  • The presented spectral and frequency encoding method offers a promising approach for high-resolution fluorescence imaging.
  • This technique has significant potential for applications in advanced endoscopic devices.